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KMID : 0350519920450041321
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Journal of Catholic Medical College
1992 Volume.45 No. 4 p.1321 ~ p.1334
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Production and Characterization of Monoclonal Antibody, C536, Reactive with Human Squamous Cell Carcinoma of the Uterine Cervix
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Abstract
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Murine monoclonal antibodies were produced by fusion of spleen cells obtained from mice immunized with CUMC-6, a human cell line of squamous Cell carcinoma derived from uterine cervix, and P3-*63-Ag8 myeloma cells.
We obtained 51 hybridomas secreting specific monoclonal antibodies to cervical carcinoma antigen continuously.
Among them, one hybridoma designated C536 that was highly reactive with cervical carcinoma was selected, and examined on the staining pattern and the reactivity with antigenic determinants of cervical carcinoma.
@ES The results were as follows:
@EN 1. Immunohistochemical staining revealed that C536 monoclonal antibody reacted with frozen tissue sections of cervical carcinoma tissues (90% diagnostic accuracy rate), but not with ovarian, and endometrial carcinomas.
2. The isotype and subclass of C536 monoclanal antibody was IgG2b in hemagglutination assay.
3. Sodium dodecyl sulfate polyacrylamide gel electrophoretic (SDS-PAGE) analysis of C536 monoclonal antibody immunoprecipitates of extracts of L-[35S] methionine-labeled human cervical carcinoma cells(HeLa, CuMC-6) showed a major band in
apparent
molecular weight, 51,000 daltons.
All these data are in favor of the use of C536 monoclonal antibody as an useful immunodiagnostic tool for cervical carcinoma.
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